Journal: Biophysical Journal

Article Title: Triplet Imaging of Oxygen Consumption during the Contraction of a Single Smooth Muscle Cell (A7r5)

doi: 10.1016/j.bpj.2009.10.006

Figure Lengend Snippet: Three experiments on contraction of smooth muscle cells A7r5 upon addition of AVP and two negative controls where no AVP was added. (A) Average triplet relaxation rate measured on a small area inside the respective cells. (B) Relaxation rates normalized with respect to the initial and final steady state. For the negative controls we used a factor of 0.4 × 10−5 for the normalization, corresponding to a typical difference between the steady-state values before and after adding AVP. Global fitting was used to determine the respective intersystem crossing rates τisc leading to the following values: (cell 1) 2.12 μs; (cell 2) 2.01 μs; (cell 3) 1.19 μs; (cell 4) 1.08 μs; and (cell 5) 2.01 μs. See Movie S1, Movie S2, and Movie S3 for depictions of the three experiments.

Article Snippet: For biological measurements, we used the rat thoracic aorta smooth muscle cell line A7r5 ( 44 ) (cat. No. CRL-1444; LGC Promochem, Wesel, Germany) cultured in Dulbecco's modified eagle medium (DMEM, cat. No. 41965; GIBCO, Invitrogen) supplemented with 10% fetal calf serum (FCS).

Techniques:

Journal: Biophysical Journal

Article Title: Triplet Imaging of Oxygen Consumption during the Contraction of a Single Smooth Muscle Cell (A7r5)

doi: 10.1016/j.bpj.2009.10.006

Figure Lengend Snippet: Two experiments on contraction of smooth muscle cells A7r5 with transient transfections of the cytosolic fusion protein β-galactosidase (SNAP-β-gal) employed for the labeling with TMR. Contraction was initiated with [Arg8]-vasopressin (AVP). (A2 and B2) Triplet lifetime image with color-encoded lifetime. (A3 and B3) Temporal evolution of the average triplet relaxation rate for the region inside the white rectangle. The result indicates a variation in the oxygen concentration after a monoexponential decay (the approximate decay parameter was estimated from multiple experiments). We used (A) τisc = 2.12 μs and (B) τisc = 2.01 μs; both values were obtained by global fitting on several measurements. The image quality was optimized for multiple measurements. A typical triplet lifetime image with higher spatial resolution can be found in the Supporting Material.

Article Snippet: For biological measurements, we used the rat thoracic aorta smooth muscle cell line A7r5 ( 44 ) (cat. No. CRL-1444; LGC Promochem, Wesel, Germany) cultured in Dulbecco's modified eagle medium (DMEM, cat. No. 41965; GIBCO, Invitrogen) supplemented with 10% fetal calf serum (FCS).

Techniques: Transfection, Labeling, Concentration Assay

Journal: Scientific Reports

Article Title: Novel 1, 4-dihydropyridines for L-type calcium channel as antagonists for cadmium toxicity

doi: 10.1038/srep45211

Figure Lengend Snippet: Calcium overload-preventing activity (IC 50 ) of novel synthesized compounds in A7r5 and SH-SY5Y cells.

Article Snippet: The A7r5 (rat aorta smooth muscle cells) and SH-SY5Y (human neuroblastoma cell line) were obtained from the National Centre for Cell Science, Pune, India.

Techniques: Activity Assay, Synthesized

Journal: Scientific Reports

Article Title: Novel 1, 4-dihydropyridines for L-type calcium channel as antagonists for cadmium toxicity

doi: 10.1038/srep45211

Figure Lengend Snippet: The effect of a 1 hr Amlodipine (AD) pre-treatment on CdCl 2 treated A7r5 cells represented curve with purple color. The effect of a 1 h 5a compound pre-treatment on CdCl 2 treated A7r5 cells represented curve with green color. The effect of a 1 hr 5e compound pre-treatment onCdCl 2 treated A7r5 cells represented curve with yellow color.

Article Snippet: The A7r5 (rat aorta smooth muscle cells) and SH-SY5Y (human neuroblastoma cell line) were obtained from the National Centre for Cell Science, Pune, India.

Techniques: